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KMID : 0606920070150040205
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Biomolecules & Therapeutics
2007 Volume.15 No. 4 p.205 ~ p.211
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Neuroprotective and Antioxidant Effects of the Butanol Fraction Prepared from Opuntia ficus-indica var. saboten
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Cho Jung-Sook
Jin Chang-Bae
Lee Yong-Sup
Han Chang-Kyun
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Abstract
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The fruits and stems of Opuntia ficus-indica var. saboten have been reported to exhibit a variety of
pharmacological actions, including antioxidant, analgesic, anti-inflammatory, and anti-ulcer effects. In the present
study, we evaluated effects of the butanol fraction (SK OFB901) prepared from the 50% ethanol extract of the
stems on various types of neuronal injuries induced by oxidative stress, excitotoxins, and amyloid β (Aβ) in primary
cultured rat cortical cells. Its antioxidant and radical scavenging activities were also evaluated by cell-free
bioassays. We found that SK OFB901 strongly inhibited the oxidative neuronal damage induced by H2O2 or xanthine/
xanthine oxidase. In addition, it exhibited marked inhibition of the excitotoxic neuronal damage induced by
glutamate, N-methyl-D-aspartic acid, or kainate. Furthermore, the Aβ(25-35)-induced neurotoxicity was also significantly
attenuated by SK OFB901. It was found to inhibit lipid peroxidation initiated by Fe2+ and L-ascorbic
acid in rat brain homogenates and scavenge 1,1-diphenyl-2-picrylhydrazyl free radicals. These results indicate
that the butanol fraction prepared from the stems of Opuntia ficus-indica var. saboten exerts potent antioxidant
and neuroprotective effects through multiple mechanisms, implying its potential applications for the prevention or
management of neurodegenerative disorders associated with oxidative stress, excitotoxicity, and Aβ.
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KEYWORD
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Opuntia ficus-indica, Neuroprotection, Antioxidant, Cortical culture, Excitotoxicity, Amyloid ${\beta}\, (A{\beta})$
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